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Anti human Nanog antibody
Anti human Nanog antibody
產品編號: RCAB004P-F
規  格:
單  價: 詢價
生 產 商: ReproCELL
到貨時間: 4-6weeks
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Transcriptional factors, OCT3/4 (POU5F1) and STAT3 function as key regulators in maintaining pluripotency of stem cells. Thus, POU5F1 and STAT3 have been widely used as molecular markers of pluripotential stem cells. Pluripotential cell-specific Nanog gene is a newly identified homeodomain-bearing transcriptional factor. Importantly, Nanog is expressed specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Therefore, Nanog is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human.

human Nanog antibody
Immunogen Human Nanog peptide
Raised in Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified
Species specificity Human specific
Cross reactivity No cross reactivity
Concentration 0.20 mg/mL
Tested applications Western blot
Immunocytochemistry
Application notes WB: Use at a dilution of 1/300 – 1/2000
Immunocytochemistry: Use at a dilution of 1/150 – 1/700
Storage buffer Phosphate buffered saline incl. 0.1% sodium azide
Storage Store at +4°C for short term use. Aliquot and store at -20°C or -80°C.
Avoid repeated freeze / thaw cycles.

 

human Nanog antibody immunostaining

IF using hNanog antibody
Cells: Human embryonic stem cells, grown on mouse embryonic fibroblasts.

human Nanog antibody western blot

Western blot using hNanog antibody

 


[1]Du, Dongshu, and Xiangxin Lou. “Generation of induced pluripotent stem cells from neonatal mouse cochlear cells.” Differentiation (2014).
[2]Inada, Yutaka, et al. “Cell and tissue-autonomous development of the circadian clock in mouse embryos.” FEBS letters 588.3 (2014): 459-465.
[3]Izumikawa, Tomomi, Ban Sato, and Hiroshi Kitagawa. “Chondroitin Sulfate Is Indispensable for Pluripotency and Differentiation of Mouse Embryonic Stem Cells.” Scientific reports 4 (2014).
[4]Kowno, Megumi, et al. “Prohibitin 2 Regulates the Proliferation and Lineage-Specific Differentiation of Mouse Embryonic Stem Cells in Mitochondria.” PloS one 9.4 (2014): e81552.
[5]Sangel, Percival, Masahiro Oka, and Yoshihiro Yoneda. “The Role of Importin-βs in the Maintenance and Lineage Commitment of Mouse Embryonic Stem Cells.” FEBS Open Bio (2014).
[6]Yamasaki, Sachiko, et al. “Generation of Human Induced Pluripotent Stem (Ips) Cells in Serum-and Feeder-Free Defined Culture and TGF-Β1 Regulation of Pluripotency.” PLOS ONE 9.1 (2014): e87151.
[7]Araki, Ryoko, et al. “Negligible immunogenicity of terminally differentiated cells derived from induced pluripotent or embryonic stem cells.” Nature (2013).
[8]Donai, Kenichiro, et al. “Establishment of a reporter system to monitor silencing status in induced pluripotent stem cell lines.” Analytical biochemistry 443.1 (2013): 104-112.

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